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Digital
Video Database
Digitize
Your Lecture
Create
a custom DVD to accompany your lab manual. Our DVD database
includes video on lab safety, microscope use, Gram staining,
pH testing, pipetting, diffusion and osmosis, respirometer
use, and more. Video clips can be combined in any order
to match your lab manual, or customized with new video created
just for you. Adding content to existing DVD material or
writing new DVD script can earn you, your department, or
your school royalties. The professional videographers at
Bent Tree Press can shoot video at your location, or use
our biologists to shoot video for you—all at incredible
cost-saving pricing!
VIEW
CLIPS OF OUR DVD VIDEOS:
Microbiology
- Lab
Safety
Covers
basic lab safety, including use of fire extinguisher and
fire blanket, location of First Aid kit, use of eyewash
station, disinfection of workstation, disposal of sharp
and contaminated materials in the SHARPS and biohazard
containers, and location of MSDS information.
-
Parts
of the Microscope
Identifies
and discusses uses of the ocular eyepieces, diopter ring,
rotating nosepiece, objective lenses, stage, condenser,
condensure apeture diaphragm, daylight filter, base, light
source, X & Y travel controls, fine and course focus
knobs, arms, and power switch.
- Use
of the Microscope
Shows
proper transport and setup of microscope, cleaning of
ocular and objective lenses, placement of slide, magnification,
adjustment of eyepiece plates, proper focusing procedure,
and oil immersion viewing and focus, as well as microscope
cleanup and storage.
- Aseptic
Technique
Covers
labeling a culture, turning on and adjusting the Bunsen
burner, flaming the loop, transferring organisms from
a broth, and inoculating an Agar slant.
- Smear
Preparation
Demonstrates
the steps in making a bacterial smear from both broth
and Agar, including aseptic technique for obtaining and
transferring bacteria to a slide, drying a slide, and
properly heat-fixing a slide.
- Simple
Stain
Shows
how to stain a prepared slide and provides examples of
how a correctly stained slide looks under oil immersion,
as compared to stains that are too thick or too thin.
Discusses how to avoid sampling error, and provides examples
of proper interpretation of a stain.
- The
Gram Stain
Provides
history of the Gram stain and discusses positive and negative
results obtained from the procedure. Shows the 4 reagents
used in the Gram stain, and discusses their roles in the
staining process, as well as how to avoid sampling error.
Demonstrates the steps of the staining procedure and shows
examples of correctly stained slides, as well as interpretation
of slides.
- Streak
Plate Technique
Discusses
reasons for using dilution technique and shows labeling,
T-streaking, examples of properly and improperly streaked
cultures, and how to avoid sampling error.
- Serial
Dilutions Using Serological Pipettes
Shows
the setup and labeling of test tubes, aseptic technique
for obtaining a pipette, attaching a pipetting device,
aspirating liquid, filling test tubes with water and broth,
mixing and serial transferring of liquid from one tube
to another, and disposing of used pipettes. Shows labeling
of sterile Petri dishes, transferring broth from test
tubes to culture dishes, and steps in proper spread plate
technique.
- Serial
Dilutions Using Micropipettors
Shows
setup and labeling of microcentrifuge tubes, and micropipetting
of water and broth into each tube. Demonstrates mixing
and serial transferring of liquid from one tube to another.
Shows labeling of sterile Petri dishes, transferring of
broth from test tubes to culture dishes, and proper spread
plate technique.
Biology
- Using
the Computer
Shows
how to turn on the Apple eMac computer, log in, and gain
Internet access. Discusses how to obtain course information
and use weekly links buttons for Web-based lab information.
Shows how to turn off the computer at the end of lab.
- Safety
Discusses
lab safety rules for food and clothing, lab procedures
for backpacks and phones, emergency phone and phone number
location in the lab, as well as eyewash station location
and use, First Aid kit location, fire extinguisher location
and use, and biohazard material disposal.
- Introduction
to the Microscope
Shows
proper transport and setup of the microscope, and identifies
the oculars, stage, objectives, course and fine focus
knobs, slide clip, stage manipulators, power switch, dimmer
dial, and iris.
- Using
the Microscope
Demonstrates how to place a slide, adjust the
objective lenses and eyepieces, focus under low power,
increase magnification, and adjust with the fine focus.
Shows proper technique for preparing the microscope for
storage and transporting it back to the shelf.
- Obsertainer
Discusses what an obsertainer box is, where to
locate the box number, and how to use the obsertainer
in lab.
- Cheek
Cell Preparation
Shows how to clean and prepare a microscope slide,
apply methylene blue dye, obtain a cheek cell scraping,
apply a coverslip to avoid bubbles, dispose of the used
coverslip, and clean and store the slide for future use.
- pH
Testing
Discusses pH and the pH scale, and shows how
to test a sample and read a pH strip.
- Cell
Chemistry: The Test for the Presence of Starch
Demonstrates how iodine reacts with starch in
a potato, and shows how to prepare test tubes and unknowns
for the presence of starch and two other biomolecules.
Shows how to setup and conduct the experiment for the
starch assay and how to perform the Benedict’s test
for sugar and the Biuret test for protein.
- Enzymes
Shows how to prepare and label a spot plate for
the amylase enzyme test. Demonstrates how to add iodine
to each well and test for starch breakdown in the wells
over time. Shows how to time and take samples and read
results, including negative and positive controls. Discusses
variation in results due to heating, cooling, diluting,
or changing pH.
- pH
Effect on Enzymes
Shows the effect of exposing the enzyme amylase
to acid, and demonstrates the spot plate test with the
altered enzyme.
- Diffusion
and Osmosis
Discusses the movement of molecules by diffusion
and osmosis and demonstrates the effect with red dye,
water, and temperature. Demonstrates how to prepare dialysis
tubing with soaking, folding, clipping, and filling. Shows
how to tare the dialysis tubing and perform the experiment.
- Cellular
Respiration: Respirometers
Shows how to make a respirometer by filling and
inverting two differently sized test tubes. Demonstrates
how to prepare three tubes for the experiment, including
the negative and positive controls, by adding the yeast,
water, and glucose to the tubes. Shows the instructor
adding the toxic sodium azide and the addition of phenol
red. Demonstrates inversion of the experimental respirometers.
- Fermentation
Tubes
Demonstrates the steps for testing the effectiveness
of various respiratory fuels, and shows proper technique
for working with a fermentation tube.
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